Microflow liquid chromatography coupled to mass spectrometry--an approach to significantly increase sensitivity, decrease matrix effects, and reduce organic solvent usage in pesticide residue analysis.
Identifieur interne : 000544 ( Main/Exploration ); précédent : 000543; suivant : 000545Microflow liquid chromatography coupled to mass spectrometry--an approach to significantly increase sensitivity, decrease matrix effects, and reduce organic solvent usage in pesticide residue analysis.
Auteurs : Ana Uclés Moreno [Espagne] ; Sonia Herrera L Pez ; Barbara Reichert ; Ana Lozano Fernández ; María Dolores Hernando Guil ; Amadeo Rodríguez Fernández-AlbaSource :
- Analytical chemistry [ 1520-6882 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Pesticide Residues.
- chemistry : Capsicum, Citrus sinensis, Lycopersicon esculentum, Solvents.
- methods : Chromatography, Liquid, Solid Phase Extraction, Spectrometry, Mass, Electrospray Ionization.
- Sensitivity and Specificity.
Abstract
This manuscript reports a new pesticide residue analysis method employing a microflow-liquid chromatography system coupled to a triple quadrupole mass spectrometer (microflow-LC-ESI-QqQ-MS). This uses an electrospray ionization source with a narrow tip emitter to generate smaller droplets. A validation study was undertaken to establish performance characteristics for this new approach on 90 pesticide residues, including their degradation products, in three commodities (tomato, pepper, and orange). The significant benefits of the microflow-LC-MS/MS-based method were a high sensitivity gain and a notable reduction in matrix effects delivered by a dilution of the sample (up to 30-fold); this is as a result of competition reduction between the matrix compounds and analytes for charge during ionization. Overall robustness and a capability to withstand long analytical runs using the microflow-LC-MS system have been demonstrated (for 100 consecutive injections without any maintenance being required). Quality controls based on the results of internal standards added at the samples' extraction, dilution, and injection steps were also satisfactory. The LOQ values were mostly 5 μg kg(-1) for almost all pesticide residues. Other benefits were a substantial reduction in solvent usage and waste disposal as well as a decrease in the run-time. The method was successfully applied in the routine analysis of 50 fruit and vegetable samples labeled as organically produced.
DOI: 10.1021/ac5035852
PubMed: 25495653
Affiliations:
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Le document en format XML
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Sacramento, s/n, 04120 La Cañada de San Urbano, Almerı́a, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Agrifood Campus of International Excellence (ceiA3), European Union Reference Laboratory for Pesticide Residues in Fruit & Vegetables, University of Almeria , Ctra. 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This uses an electrospray ionization source with a narrow tip emitter to generate smaller droplets. A validation study was undertaken to establish performance characteristics for this new approach on 90 pesticide residues, including their degradation products, in three commodities (tomato, pepper, and orange). The significant benefits of the microflow-LC-MS/MS-based method were a high sensitivity gain and a notable reduction in matrix effects delivered by a dilution of the sample (up to 30-fold); this is as a result of competition reduction between the matrix compounds and analytes for charge during ionization. Overall robustness and a capability to withstand long analytical runs using the microflow-LC-MS system have been demonstrated (for 100 consecutive injections without any maintenance being required). Quality controls based on the results of internal standards added at the samples' extraction, dilution, and injection steps were also satisfactory. The LOQ values were mostly 5 μg kg(-1) for almost all pesticide residues. Other benefits were a substantial reduction in solvent usage and waste disposal as well as a decrease in the run-time. The method was successfully applied in the routine analysis of 50 fruit and vegetable samples labeled as organically produced.</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><noCountry><name sortKey="Fernandez Alba, Amadeo Rodriguez" sort="Fernandez Alba, Amadeo Rodriguez" uniqKey="Fernandez Alba A" first="Amadeo Rodríguez" last="Fernández-Alba">Amadeo Rodríguez Fernández-Alba</name><name sortKey="Hernando Guil, Maria Dolores" sort="Hernando Guil, Maria Dolores" uniqKey="Hernando Guil M" first="María Dolores" last="Hernando Guil">María Dolores Hernando Guil</name><name sortKey="Herrera L Pez, Sonia" sort="Herrera L Pez, Sonia" uniqKey="Herrera L Pez S" first="Sonia" last="Herrera L Pez">Sonia Herrera L Pez</name><name sortKey="Lozano Fernandez, Ana" sort="Lozano Fernandez, Ana" uniqKey="Lozano Fernandez A" first="Ana" last="Lozano Fernández">Ana Lozano Fernández</name><name sortKey="Reichert, Barbara" sort="Reichert, Barbara" uniqKey="Reichert B" first="Barbara" last="Reichert">Barbara Reichert</name></noCountry><country name="Espagne"><noRegion><name sortKey="Ucles Moreno, Ana" sort="Ucles Moreno, Ana" uniqKey="Ucles Moreno A" first="Ana" last="Uclés Moreno">Ana Uclés Moreno</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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